Aiming at the problem of influence of the channel on the characteristics of the vowel formant, a systematic experiment was carried out. Firstly, the standard recordings of 8 volunteers were collected. Then, the standard recordings were played with the mouth simulator, and 104 channel recordings were recorded using 13 different channels. Finally, the characteristic voice segments were extracted, and chi-square test analysis was used in the qualitative analysis of the spectral characteristics, and one-sample t-test was used in the quantitative analysis of acoustic parameters. The statistical results show that about 69% of the channels have a significant influence on the overall form of the high-order formants, and about 85% of the channels have significant differences in the relative intensity of the formants. The one-sample t-test results show that there is no significant difference between the standard recordings and the channel recordings in center frequency of the formant. Experimental results show that the frequency characteristics of formants should be paid more attention to when processing the identification of voices in different channels.